Protein identification (from 1-DE, 2-DE gels and liquid)
The dream of having the complete genome sequence is now a reality. The complete sequence of several genomes including the human one is known. However, the understanding of probably half a million human proteins encoded by some 23'000 genes is still a long way away and the hard work to unravel the complexity of biological systems is yet to come.
The heart of this new approach is the simultaneous separation of thousand of polypeptides using either electrophoresis or liquid chromatography techniques their further identification combining high-throughput mass spectrometry analysis to powerful bioinformatic tools. The Geneva Proteomics Core Facility provides 3 identification services to which you may send your samples for analysis. These services include tryptic digestion from 1-DE, 2-DE and liquid samples. The peptide mixtures can be then analysed by three alternative workflows: Maldi Tof-Tof, NanoLC - Maldi Tof-Tof and NanoLC - ESI-MS/MS.
Sample preparation
Gel: The sample preparation, gel separation and gel staining have to be performed according to the SWISS-2DPAGE protocols. Put your gel piece into an Eppendorf tube in 50µl of ethanol 10% and identify your sample with a water-resistant pencil.
Liquid: For liquid samples Contact us to know how to prepare the samples. Put your liquid sample into an Eppendorf tube and clearly identify your sample with a water-resistant pencil.
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