Molecular biology
VIRION
Enveloped, spherical. Diameter from about 150nm nm.
GENOME
Negative-stranded RNA linear genome, about 15 kb in size. Encodes for eight proteins.
GENE EXPRESSION
The viral RNA dependent RNA polymerase binds the encapsidated genome at the leader region, then sequentially transcribes each genes by recognizing start and stop signals flanking viral genes. mRNAs are capped and polyadenylated by the L protein during synthesis.
REPLICATION
CYTOPLASMIC
- Virus attaches to host receptors though GP glycoproteins and is endocytosed into vesicles in the host cell.
- Fusion of virus membrane with the vesicle membrane; ribonucleocapsid is released in the cytoplasm.
- sequential transcription, viral mRNAs are capped and polyadenylated in the cytoplasm.
- Replication presumably starts when enough necleoprotein is present to encapsidate neo-synthetized antigenomes and gemomes.
- the ribonucleocapsid interacts with the matrix protein under the plasma membrane and buds, releasing the virion.